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pentr tre vector  (Addgene inc)


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    Structured Review

    Addgene inc pentr tre vector
    Pentr Tre Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pslik+neo+vectors/pSLIK-Neo+(Plasmid+%2325735)/pmc12782853-61-17-32
    Average 93 stars, based on 45 article reviews
    pentr tre vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Produced:

    Article Title: ALS-associated RNA binding proteins converge on UNC13A transcription through regulation of REST
    Article Snippet: For doxycycline-inducible FLAG-FUS (WT and mutant) or 3×FLAG-hnRNPA1 expression in corresponding KO cell lines, the FUS-KO or hnRNPA1-KO cells were infected with lentiviruses in the presence of polybrene (8 μg/ml) and then subjected to selection with G418 (400 μg/ml, Wako) for at least 7 days. .. The lentiviruses were produced by transfection of HEK293T cells with the pSLIK-neo vectors containing the rtTA-TRE–regulated FLAG-FUS or 3×FLAG-hnRNPA1 cDNA sequences, as well as with the packaging plasmid psPAX2 and the envelope plasmid pMD2.G (Addgene). .. For doxycycline-inducible MATR3 expression in MATR3-KO cells, the cells were transfected with the corresponding PiggyBac vector and the Super PiggyBac Transposase Expression Vector (System Biosciences) and were then subjected to selection with G418 (400 μg/ml) for 2 weeks.

    Transfection:

    Article Title: ALS-associated RNA binding proteins converge on UNC13A transcription through regulation of REST
    Article Snippet: For doxycycline-inducible FLAG-FUS (WT and mutant) or 3×FLAG-hnRNPA1 expression in corresponding KO cell lines, the FUS-KO or hnRNPA1-KO cells were infected with lentiviruses in the presence of polybrene (8 μg/ml) and then subjected to selection with G418 (400 μg/ml, Wako) for at least 7 days. .. The lentiviruses were produced by transfection of HEK293T cells with the pSLIK-neo vectors containing the rtTA-TRE–regulated FLAG-FUS or 3×FLAG-hnRNPA1 cDNA sequences, as well as with the packaging plasmid psPAX2 and the envelope plasmid pMD2.G (Addgene). .. For doxycycline-inducible MATR3 expression in MATR3-KO cells, the cells were transfected with the corresponding PiggyBac vector and the Super PiggyBac Transposase Expression Vector (System Biosciences) and were then subjected to selection with G418 (400 μg/ml) for 2 weeks.

    Plasmid Preparation:

    Article Title: ALS-associated RNA binding proteins converge on UNC13A transcription through regulation of REST
    Article Snippet: For doxycycline-inducible FLAG-FUS (WT and mutant) or 3×FLAG-hnRNPA1 expression in corresponding KO cell lines, the FUS-KO or hnRNPA1-KO cells were infected with lentiviruses in the presence of polybrene (8 μg/ml) and then subjected to selection with G418 (400 μg/ml, Wako) for at least 7 days. .. The lentiviruses were produced by transfection of HEK293T cells with the pSLIK-neo vectors containing the rtTA-TRE–regulated FLAG-FUS or 3×FLAG-hnRNPA1 cDNA sequences, as well as with the packaging plasmid psPAX2 and the envelope plasmid pMD2.G (Addgene). .. For doxycycline-inducible MATR3 expression in MATR3-KO cells, the cells were transfected with the corresponding PiggyBac vector and the Super PiggyBac Transposase Expression Vector (System Biosciences) and were then subjected to selection with G418 (400 μg/ml) for 2 weeks.



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